Proteintech, 12844-1-AP, FBXO17 Polyclonal antibody

Size: 20ul / 150ul FBXO17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA 12844-1-AP targets FBXO17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: U-251 cells, mouse heart tissue, HepG2 cells, mouse lung tissue Positive IHC detected in: human heart tissue, human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information FBXO17, also named as FBG4, FBX17, FBX26 and FBXO26, is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3867 Product name: Recombinant human FBXO17 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-278 aa of BC012385 Sequence: MGARLSRRRLPADPSLALDALPPELLVQVLSHVPPRSLVTRCRPVCRAWRDIVDGPTVWLLQLARDRSAEGRALYAVAQRCLPSNEDKEEFPLCALARYCLRAPFGRNLIFNSCGEQGFRGWEVEHGGNGWAIEKNLTPVPGAPSQTCFVTSFEWCSKRQLVDLVMEGVWQELLDSAQIEICVADWWGARENCGCVYQLRVRLLDVYEKEVVKFSASPDPVLQWTERGCRQVSHVFTNFGKGIRYVSFEQYGRDVSSWVGHYGALVTHSSVRVRIRLS Predict reactive species Full Name: F-box protein 17 Calculated Molecular Weight: 278 aa, 31 kDa Observed Molecular Weight: 29-31 kDa GenBank Accession Number: BC012385 Gene Symbol: FBXO17 Gene ID (NCBI): 115290 RRID: AB_2877889 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96EF6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.