Proteintech, 12857-1-AP, STK33 Polyclonal antibody

Size: 20ul / 150ul The STK33 (12857-1-AP) by Proteintech is a Polyclonal antibody targeting STK33 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 12857-1-AP targets STK33 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human kidney tissue, mouse testis tissue Positive IP detected in: HEK-293 cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Serine/threonine kinase 33 (STK33) gene, which is identified in human chromosome 11 and mouse chromosome 7, encodes a serine/threonine kinase. STK33 belongs to a member of the calcium/calmodulin‐dependent kinase (CAMK), which is mainly existed in testis, certain brain regions and embryonic organs such as brain, heart and spinal cord in human and mouse and is likely to participate in spermatogenesis and organ ontogenesis. (PMID: 30256516, PMID: 34536775) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3561 Product name: Recombinant human STK33 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-381 aa of BC031231 Sequence: MADSGLDKKSTKCPDCSSASQKDVLCVCSSKTRVPPVLVVEMSQTSSIGSAESLISLERKKEKNINRDITSRKDLPSRTSNVERKASQQQWGRGNFTEGKVPHIRIENGAAIEEIYTFGRILGKGSFGIVIEATDKETETKWAIKKVNKEKAGSSAVKLLEREVNILKSVKHEHIIHLEQVFETPKKMYLVMELCEDGELKEILDRKGHFSENETRWIIQSLASAIAYLHNNDIVHRDLKLENIMVKSSLIDDNNEINLNIKVTDFGLAVKKQSRSEAMLQATCGTPIYMAPEVISAHDYSQQCDIWSIGVVMYMLLRGEPPFLASSEEKLFELIRKGELHFENAVWNSISDCAKSVLKQLMKVDPAHRITAKELLDNQWL Predict reactive species Full Name: serine/threonine kinase 33 Calculated Molecular Weight: 514 aa, 58 kDa Observed Molecular Weight: 53 kDa GenBank Accession Number: BC031231 Gene Symbol: STK33 Gene ID (NCBI): 65975 RRID: AB_10642563 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BYT3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.