Proteintech, 12894-1-AP, Ribophorin I Polyclonal antibody

Size: 20ul / 150ul The Ribophorin I (12894-1-AP) by Proteintech is a Polyclonal antibody targeting Ribophorin I in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 12894-1-AP targets Ribophorin I in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HT-29 cells, K-562 cells, HeLa cells Positive IHC detected in: mouse lung tissue, rat liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Ribophorin I, also named as RPN1, is a key protein component of the oligosaccharyltransferase (OST) complex, which is embedded in the membrane of the rough endoplasmic reticulum (RER) (PMID: 38373565). It plays a crucial role in the process of N-linked glycosylation, a post-translational modification essential for the proper folding, stability, and function of many secretory and membrane-bound proteins (PMID: 17264154). Ribophorin I, along with Ribophorin II, forms a heterodimeric complex that facilitates the transfer of oligosaccharides from a lipid-linked donor to specific asparagine residues on nascent polypeptide chains (PMID: 30940778). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, yeast Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3576 Product name: Recombinant human RPN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 22-350 aa of BC010839 Sequence: GSASSEAPPLINEDVKRTVDLSSHLAKVTAEVVLAHLGGGSTSRATSFLLALEPELEARLAHLGVQVKGEDEEENNLEVRETKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYSQDTFKVHYENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISSIRSFKTILPAAAQDVYYRDEIGNVSTSHLLILDDSVEMEIRPRFPLFGGWKTHYIVGYNLPSYEYLYNLGDQYA Predict reactive species Full Name: ribophorin I Calculated Molecular Weight: 69 kDa Observed Molecular Weight: 66-69 kDa GenBank Accession Number: BC010839 Gene Symbol: RPN1 Gene ID (NCBI): 6184 RRID: AB_2170474 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P04843 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.