Proteintech, 12911-1-AP, AOAH Polyclonal antibody

Size: 20ul / 150ul The AOAH (12911-1-AP) by Proteintech is a Polyclonal antibody targeting AOAH in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 12911-1-AP targets AOAH in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HL-60 cells, mouse colon tissue, mouse spleen tissue, THP-1 cells Positive IHC detected in: mouse colon tissue, human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Acyloxyacyl hydrolase (AOAH) is a highly conserved lipase responsible for hydrolyzing the secondary fatty acyl chains of gram-negative bacterial lipopolysaccharides (LPS), resulting in LPS detoxification and attenuation of host inflammation (PMID: 1577781, PMID: 35980963). AOAH has also been shown to have PLA2 function (PMID: 1577781). AOAH was highly expressed at the protein level in lesional psoriatic skin compared to healthy controls (PMID: 34913478). AOAH has been described to be differentially proteolytically processed into both a secreted and cytosolic form in phagocytic cells (PMID: 19755182). Expression of AOAH is primarily attributed to phagocytic cells such as neutrophils, macrophages, DC, and also NK cells (PMID: 33454018). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3892 Product name: Recombinant human AOAH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 226-575 aa of BC025698 Sequence: QDSNCNGIWGVDPKDGVPYEKKFCEGSQPRGIILLGDSAGAHFHISPEWITASQMSLNSFINLPTALTNELDWPQLSGATGFLDSTVGIKEKSIYLRLWKRNHCNHRDYQNISRNGASSRNLKKFIESLSRNKVLDYPAIVIYAMIGNDVCSGKSDPVPAMTTPEKLYSNVMQTLKHLNSHLPNGSHVILYGLPDGTFLWDNLHNRYHPLGQLNKDMTYAQLYSFLNCLQVSPCHGWMSSNKTLRTLTSERAEQLSNTLKKIAASEKFTNFNLFYMDFAFHEIIQEWQKRGGQPWQLIEPVDGFHPNEVALLLLADHFWKKVQLQWPQILGKENPFNPQIKQVFGDQGGH Predict reactive species Full Name: acyloxyacyl hydrolase (neutrophil) Calculated Molecular Weight: 65 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC025698 Gene Symbol: AOAH Gene ID (NCBI): 313 RRID: AB_2877891 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P28039 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.