Proteintech, 12923-1-AP, NME5 Polyclonal antibody

Size: 20ul / 150ul The NME5 (12923-1-AP) by Proteintech is a Polyclonal antibody targeting NME5 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 12923-1-AP targets NME5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, human kidney tissue Positive IP detected in: mouse testis tissue Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information NME5(Nucleoside diphosphate kinase homolog 5) belongs to the NDK family. Its expression plays a critical role in spermiogenesis by increasing the cellular levels of GPX5 to eliminate reactive oxygen species. NME5 may have specific functions in the phosphotransfer network involved in spermiogenesis and flagellar movement. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3610 Product name: Recombinant human NME5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-212 aa of BC026182 Sequence: MEISMPPPQIYVEKTLAIIKPDIVDKEEEIQDIILRSGFTIVQRRKLRLSPEQCSNFYVEKYGKMFFPNLTAYMSSGPLVAMILARHKAISYWLELLGPNNSLVAKETHPDSLRAIYGTDDLRNALHGSNDFAAAEREIRFMFPEVIVEPIPIGQAAKDYLNLHIMPTLLEGLTELCKQKPADPLIWLADWLLKNNPNKPKLCHHPIVEEPY Predict reactive species Full Name: non-metastatic cells 5, protein expressed in (nucleoside-diphosphate kinase) Calculated Molecular Weight: 212 aa, 24 kDa Observed Molecular Weight: 24 kDa GenBank Accession Number: BC026182 Gene Symbol: NME5 Gene ID (NCBI): 8382 RRID: AB_2152973 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P56597 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.