Proteintech, 12965-1-AP, ACP6 Polyclonal antibody

Size: 20ul / 150ul The ACP6 (12965-1-AP) by Proteintech is a Polyclonal antibody targeting ACP6 in WB, IHC, ELISA applications with reactivity to human, mouse samples 12965-1-AP targets ACP6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A2780 cells, mouse liver tissue, COLO 320 cells, HepG2 cells, RT-4 cells, SKOV-3 cells Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Lysophosphatidic acid phosphatase type 6 (ACP6) is an LPA-specific acid phosphatase that hydrolyzes LPA to monoacylglycerol (MAG) and phosphate. Previous studies have shown that ACP6 is localized in the mitochondria with ubiquitous expression throughout all tissues, and high expression levels in kidney, heart, small intestine, muscle, and liver. Through specifi cally hydrolyzing LPA, ACP6 regulates mitochondrial lipid biosynthesis, thereby controlling important mitochondrial functions. It was also shown that ACP6 might be involved in tumor progression, suggesting that ACP6 could be a good candidate marker for the prognosis of esophageal cancer. Uniprot showed that by alternative splicing, ACP6 may have two kinds of shear fragments with molecular weights of 49 and 31kDa respectively. (PMID: 23807634) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3633 Product name: Recombinant human ACP6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 79-429 aa of BC034686 Sequence: QLLEVPPQTQFDYTVTNLAGGPKPYSPYDSQYHETTLKGGMFAGQLTKVGMQQMFALGERLRKNYVEDIPFLSPTFNPQEVFIRSTNIFRNLESTRCLLAGLFQCQKEGPIIIHTDEADSEVLYPNYQSCWSLRQRTRGRRQTASLQPGISEDLKKVKDRMGIDSSDKVDFFILLDNVAAEQAHNLPSCPMLKRFARMIEQRAVDTSLYILPKEDRESLQMAVGPFLHILESNLLKAMDSATAPDKIRKLYLYAAHDVTFIPLLMTLGIFDHKWPPFAVDLTMELYQHLESKEWFVQLYYHGKEQVPRGCPDGLCPLDMFLNAMSVYTLSPEKYHALCSQTQVMEVGNEE Predict reactive species Full Name: acid phosphatase 6, lysophosphatidic Calculated Molecular Weight: 428 aa, 49 kDa Observed Molecular Weight: 43 kDa GenBank Accession Number: BC034686 Gene Symbol: ACP6 Gene ID (NCBI): 51205 RRID: AB_2257842 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NPH0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.