Proteintech, 12970-1-AP, OPRL1 Polyclonal antibody

Size: 20ul / 150ul The OPRL1 (12970-1-AP) by Proteintech is a Polyclonal antibody targeting OPRL1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 12970-1-AP targets OPRL1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, SH-SY5Y cells Positive IP detected in: SH-SY5Y cells Positive IHC detected in: human gliomas tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information The OPRL1 (opioid related nociceptin receptor 1) is the G-protein coupled receptor activated by the endogenou NC (neuropeptide nociceptin) (PMID:7566152). OPRL1 is widely expressed in the central nervous system, including the hippocampal dentate gyrus, cortical areas, striatum, thalamus, and hypothalamus (PMID:19501074). OPRL1 has been found to play an essential role in cognition, and to modulate inflammation and immune responses (PMID:30152845). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4079 Product name: Recombinant human OPRL1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 71-370 aa of BC038433 Sequence: LVMYVILRHTKMKTATNIYIFNLALADTLVLLTLPFQGTDILLGFWPFGNALCKTVIAIDYYNMFTSTFTLTAMSVDRYVAICHPIRALDVRTSSKAQAVNVAIWALASVVGVPVAIMGSAQVEDEEIECLVEIPTPQDYWGPVFAICIFLFSFIVPVLVISVCYSLMIRRLRGVRLLSGSREKDRNLRRITRLVLVVVAVFVGCWTPVQVFVLAQGLGVQPSSETAVAILRFCTALGYVNSCLNPILYAFLDENFKACFRKFCCASALRRDVQVSDRVRSIAKDVALACKTSETVPRPA Predict reactive species Full Name: opiate receptor-like 1 Calculated Molecular Weight: 370 aa, 41 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC038433 Gene Symbol: OPRL1 Gene ID (NCBI): 4987 RRID: AB_10638603 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P41146 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.