Proteintech, 13037-1-AP, TRIM37 Polyclonal antibody

Size: 20ul / 150ul The TRIM37 (13037-1-AP) by Proteintech is a Polyclonal antibody targeting TRIM37 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 13037-1-AP targets TRIM37 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse brain tissue, MCF-7 cells, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: mouse testis tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells, U-87 MG cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information TRIM37 (tripartite motif containing 37) is a member of the tripartite motif (RING, B-Box, and coiled-coil domains) family . Its gene mutations result in mulibrey nanism, which is a rare, autosomal-recessive growth disorder of prenatal onset, with characteristic dysmorphic features, pericardial constriction, and hepatomegaly .TRIM37 is located on chromosome 17q23, encoding a protein with an estimated molecular weight of 130 kDa or 89kDa. (PMID: 28098873, PMID: 29940807, PMID: 14757854) Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3690 Product name: Recombinant human TRIM37 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 122-450 aa of BC036012 Sequence: GMHGGHTFKPLAEIYEQHVTKVNEEVAKLRRRLMELISLVQEVERNVEAVRNAKDERVREIRNAVEMMIARLDTQLKNKLITLMGQKTSLTQETELLESLLQEVEHQLRSCSKSELISKSSEILMMFQQVHRKPMASFVTTPVPPDFTSELVPSYDSATFVLENFSTLRQRADPVYSPPLQVSGLCWRLKVYPDGNGVVRGYYLSVFLELSAGLPETSKYEYRVEMVHQSCNDPTKNIIREFASDFEVGECWGYNRFFRLDLLANEGYLNPQNDTVILRFQVRSPTFFQKSRDQHWYITQLEAAQTSYIQQINNLKERLTIELSRTQKS Predict reactive species Full Name: tripartite motif-containing 37 Calculated Molecular Weight: 964 aa, 108 kDa Observed Molecular Weight: 130 kDa GenBank Accession Number: BC036012 Gene Symbol: TRIM37 Gene ID (NCBI): 4591 RRID: AB_10640800 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O94972 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.