Proteintech, 13047-1-AP, DDX11 Polyclonal antibody

Size: 20ul / 150ul The DDX11 (13047-1-AP) by Proteintech is a Polyclonal antibody targeting DDX11 in WB, ELISA applications with reactivity to human samples 13047-1-AP targets DDX11 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, K-562 cells, L02 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information DDX11 (DEAD/H-box helicase 11), also known as hCHLR1, KRG-2, is a member of the DExD/H (Asp-Glu-x-Asp/His)-box helicase family containing DNA helicase with roles in DNA replication and sister chromatid cohesion establishment, and general chromosome architecture (PMID: 33802088). DDX11 encodes an iron-sulfur cluster DNA helicase required for development, mutated, and overexpressed in cancers (PMID: 33879618). DDX11 is also a positive regulator of the RIG-I-mitochondrial antiviral signaling protein (MAVS)-mediated signaling pathways (PMID: 39470258). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3666 Product name: Recombinant human DDX11 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-345 aa of BC011264 Sequence: MANETQKVGAIHFPFPFTPYSIQEDFMAELYRVLEAGKIGIFESPTGTGKSLSLICGALSWLRDFEQKKREEEARLLETGTGPLHDEKDESLCLSSSCEGAAGTPRPAGEPAWVTQFVQKKEERDLVDRLKAEQARRKQREERLQQLQHRVQLKYAAKRLRQEEEERENLLRLSREMLETGPEAERLEQLESGEEELVLAEYESDEEKKVASRVDEDEDDLEEEHITKIYYCSRTHSQLAQFVHEVKKSPFGKDVRLVSLGSRQNLCVNEDVKSLGSVQLINDRCVDMQRSRHEKKKGAEEEKPKRRRQEKQAACPFYNHEQMGLLRDEALAEVKDMEQLLALGK Predict reactive species Full Name: DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae) Calculated Molecular Weight: 970 aa, 108 kDa Observed Molecular Weight: 102-108 kDa GenBank Accession Number: BC011264 Gene Symbol: DDX11 Gene ID (NCBI): 1663 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96FC9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.