Proteintech, 13050-1-AP, Claudin 1 Polyclonal antibody

Size: 20ul / 150ul The Claudin 1 (13050-1-AP) by Proteintech is a Polyclonal antibody targeting Claudin 1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, canine samples 13050-1-AP targets Claudin 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, canine samples. Tested Applications Positive WB detected in: A431 cells, mouse liver tissue, mouse skin tissue, mouse thymus tissue, A549 cells, HUVEC cells, rat skin tissue Positive IHC detected in: human cervical cancer tissue, human skin cancer tissue, mouse skin tissue, human oesophagus cancer tissue, human malignant melanoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HUVEC cells, MDCK cells, HaCaT cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Background Information Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with similar structures. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is an integral membrane protein expressed primarily in keratinocytes and normal mammary epithelial cells. Claudin 1 forms tight junctions with other claudin proteins and plays an important role in the intestinal epithelial barrier. Specification Tested Reactivity: human, mouse, rat, canine Cited Reactivity: human, mouse, rat, pig, canine, chicken, bovine, sheep, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3713 Product name: Recombinant human Claudin 1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-211 aa of BC012471 Sequence: MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV Predict reactive species Full Name: claudin 1 Calculated Molecular Weight: 211 aa, 23 kDa Observed Molecular Weight: 20-23 kDa GenBank Accession Number: BC012471 Gene Symbol: Claudin 1 Gene ID (NCBI): 9076 RRID: AB_2079881 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95832 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.