Proteintech, 13079-1-AP, PTPRA Polyclonal antibody

Size: 20ul / 150ul The PTPRA (13079-1-AP) by Proteintech is a Polyclonal antibody targeting PTPRA in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 13079-1-AP targets PTPRA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, rat liver tissue, MCF-7 cells, SKOV-3 cells Positive IP detected in: A431 cells Positive IHC detected in: human lung cancer tissue, human urothelial carcinoma tissue, mouse heart tissue, mouse kidney tissue, rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3760 Product name: Recombinant human LRP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 494-793 aa of BC027308 Sequence: LYGDTELEVTSLETHLQKIYNKIPGTSNNGLEEEFKKLTSIKIQNDKMRTGNLPANMKKNRVLQIIPYEFNRVIIPVKRGEENTDYVNASFIDGYRQKDSYIASQGPLLHTIEDFWRMIWEWKSCSIVMLTELEERGQEKCAQYWPSDGLVSYGDITVELKKEEECESYTVRDLLVTNTRENKSRQIRQFHFHGWPEVGIPSDGKGMISIIAAVQKQQQQSGNHPITVHCSAGAGRTGTFCALSTVLERVKAEGILDVFQTVKSLRLQRPHMVQTLEQYEFCYKVVQEYIDAFSDYANFK Predict reactive species Full Name: protein tyrosine phosphatase, receptor type, A Calculated Molecular Weight: 793 aa, 91 kDa Observed Molecular Weight: 90-105 kDa, 140 kDa GenBank Accession Number: BC027308 Gene Symbol: PTPRA Gene ID (NCBI): 5786 RRID: AB_2269055 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P18433 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.