Proteintech, 13129-1-AP, TrkB Polyclonal antibody

Size: 20ul / 150ul The TrkB (13129-1-AP) by Proteintech is a Polyclonal antibody targeting TrkB in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat, pig samples 13129-1-AP targets TrkB in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: mouse brain tissue, human cerebellum tissue, human stomach tissue, mouse spinal cord tissue, pig cerebellum tissue, rat brain tissue, mouse cerebellum tissue Positive IP detected in: mouse cerebellum tissue Positive IHC detected in: mouse brain tissue, mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:300-1:1200 Background Information Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance through regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes full length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416). Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse, rat, rabbit, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3790 Product name: Recombinant human NTRK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 41-433 aa of BC031835 Sequence: SRIWCSDPSPGIVAFPRLEPNSVDPENITEIFIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAHKAFLKNSNLQHINFTRNKLTSLSRKHFRHLDLSELILVGNPFTCSCDIMWIKTLQEAKSSPDTQDLYCLNESSKNIPLANLQIPNCGLPSANLAAPNLTVEEGKSITLSCSVAGDPVPNMYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVKGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLIAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYEDYGTAANDIGDTTNRSNEIPSTDVTDKTGREHLSV Predict reactive species Full Name: neurotrophic tyrosine kinase, receptor, type 2 Calculated Molecular Weight: 822 aa, 92 kDa Observed Molecular Weight: 90-95 kDa, 140-145 kDa GenBank Accession Number: BC031835 Gene Symbol: TrkB Gene ID (NCBI): 4915 RRID: AB_2155156 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q16620 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.