Proteintech, 13131-1-AP, SNTA1 Polyclonal antibody

Size: 20ul / 150ul The SNTA1 (13131-1-AP) by Proteintech is a Polyclonal antibody targeting SNTA1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 13131-1-AP targets SNTA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, rat heart tissue Positive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information SNTA1, also known as Syntrophin 1, SNT1, is cytoplasmic peripheral membrane scaffold proteins that are components of the dystrophin-associated protein complex. SNTA1 is a signal-transducing adaptor protein and serves as a scaffold for various signaling molecules. Alpha-1 syntrophin contains a PDZ domain, two Pleckstrin homology domains, and a 'syntrophin unique' domain. SNTA1 is the high expression in skeletal muscle and heart (PMID: 8576247, 8612778,). The PDZ domain of SNTA1 has been reported to bind to the C-terminal domain of murine cardiac voltage-gated sodium channels (SkM2) causing altering ion channel activity leading to Long QT syndrome(PMID 19684871, 19862833). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3820 Product name: Recombinant human SNTA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 156-505 aa of BC026215 Sequence: KKTGKEVVLEVKYMKDVSPYFKNSTGGTSVGWDSPPASPLQRQPSSPGPTPRNFSEAKHMSLKMAYVSKRCTPNDPEPRYLEICSADGQDTLFLRAKDEASARSWATAIQAQVNTLTPRVKDELQALLAATSTAGSQDIKQIGWLTEQLPSGGTAPTLALLTEKELLLYLSLPETREALSRPARTAPLIATRLVHSGPSKGSVPYDAELSFALRTGTRHGVDTHLFSVESPQELAAWTRQLVDGCHRAAEGVQEVSTACTWNGRPCSLSVHIDKGFTLWAAEPGAARAVLLRQPFEKLQMSSDDGASLLFLDFGGAEGEIQLDLHSCPKTIVFIIHSFLSAKVTRLGLLA Predict reactive species Full Name: syntrophin, alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component) Calculated Molecular Weight: 505 aa, 54 kDa Observed Molecular Weight: 54 kDa GenBank Accession Number: BC026215 Gene Symbol: SNTA1 Gene ID (NCBI): 6640 RRID: AB_3085408 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13424 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.