Proteintech, 13140-1-AP, RDH5 Polyclonal antibody

Size: 20ul / 150ul The RDH5 (13140-1-AP) by Proteintech is a Polyclonal antibody targeting RDH5 in WB, ELISA applications with reactivity to human, mouse, rat samples 13140-1-AP targets RDH5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse eye tissue, rat eye tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information RDH5, also named as RDH1, SDR9C5 and HSD17B9, belongs to short-chain dehydrogenases/reductases (SDR) family. RDH5 catalyzes the oxidation of cis-isomers of retinol in an NAD-dependent manner for the final step in the biosynthesis of 11-cis retinaldehyde. Mutations in this gene are associated with fundus albipunctatus, an autosomal recessive eye disease characterized by stationary night blindness and accumulation of white spots in the retina (PubMed:11675386). The predicted size of this protein is 35 kDa. Some paper have reported that the molecular weight is 32 kDa and could form a dimer of about 60 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3784 Product name: Recombinant human RDH5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 19-290 aa of BC028298 Sequence: RDRQSLPASNALVFITGCDSGFGRLLALQLDQRGFRVLASCLTPSGAEDLQRVASSRLHTTLLDITDPQSVQQAAKWVEMHVKEAGLFGLVNNAGVAGIIGPTPWLTRDDFQRVLNVNTMGPIGVTLALLPLLQQARGRVINITSVLGRLAANGGGYCVSKFGLEAFSDSLRRDVAHFGIRVSIVEPGFFRTPVTNLESLEKTLQACWARLPPATQAHYGGAFLTKYLKMQQRIMNLICDPDLTKVSRCLEHALTARHPRTRYSPGWDAKLL Predict reactive species Full Name: retinol dehydrogenase 5 (11-cis/9-cis) Calculated Molecular Weight: 318 aa, 35 kDa Observed Molecular Weight: 32 kDa GenBank Accession Number: BC028298 Gene Symbol: RDH5 Gene ID (NCBI): 5959 RRID: AB_2918031 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92781 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.