Proteintech, 13196-1-AP, AXL Polyclonal antibody

Size: 20ul / 150ul The AXL (13196-1-AP) by Proteintech is a Polyclonal antibody targeting AXL in WB, IP, ELISA applications with reactivity to human samples 13196-1-AP targets AXL in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: DU 145 cells, HeLa cells, HepG2 cells, human liver tissue Positive IP detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092). Specification Tested Reactivity: human Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3898 Product name: Recombinant human AXL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 545-894 aa of BC032229 Sequence: GEFGAVMEGQLNQDDSILKVAVKTMKIAICTRSELEDFLSEAVCMKEFDHPNVMRLIGVCFQGSERESFPAPVVILPFMKHGDLHSFLLYSRLGDQPVYLPTQMLVKFMADIASGMEYLSTKRFIHRDLAARNCMLNENMSVCVADFGLSKKIYNGDYYRQGRIAKMPVKWIAIESLADRVYTSKSDVWSFGVTMWEIATRGQTPYPGVENSEIYDYLRRGNRLKQPADCLDGLYALMSRCWELNPQDRPSFTELREDLENTLKALPPAQEPDEILYVNMDEGGGYPEPPGAAGGADPPTQPDPKDSCSCLTAAEVHPAGRYVLCPSTTPSPAQPADRGSPAAPGQEDGA Predict reactive species Full Name: AXL receptor tyrosine kinase Calculated Molecular Weight: 894 aa, 98 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: BC032229 Gene Symbol: AXL Gene ID (NCBI): 558 RRID: AB_10642006 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P30530 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.