Proteintech, 13214-1-AP, RPGRIP1 Polyclonal antibody

Size: 20ul / 150ul The RPGRIP1 (13214-1-AP) by Proteintech is a Polyclonal antibody targeting RPGRIP1 in WB, IHC, ELISA applications with reactivity to human, mouse samples 13214-1-AP targets RPGRIP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Y79 cells, HEK-293 cells, HL-60 cells Positive IHC detected in: mouse eye tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information RPGRIP1 is a photoreceptor protein that interacts with retinitis pigmentosa GTPase regulator protein and is a key component of cone and rod photoreceptor cells. The biological functions of RPGRIP1 are complex. Mutations in this gene lead to autosomal recessive congenital blindness. In the eye, it is expressed in amacrine neurons and photoreceptors, and in numerous other tissues, albeit at greatly reduced levels. Moreover, it exists multiple isoforms with species-specific subcellular localization patterns, and the different isoforms perform cell-specific functions. Within photoreceptors, RPGRIP1 localizes predominantly to outer segments (OS) of rods and cones in humans, whereas in bovine eyes, it is found only in rod OS. In contrast, in mice, RPGRIP1 is found primarily in connecting cilia (CC), where it localizes between the ciliary axoneme and the plasma membrane. RPGRIP1 has multiple isoforms with MW 147 kDa, 70-76 kDa, 103 kDa, and 66 kDa. Specification Tested Reactivity: human, mouse Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4089 Product name: Recombinant human RPGRIP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 320-612 aa of BC039089 Sequence: EKEHQVVSYSRRKHGKRIGVQGKNRMEYLSLNILNGNTPQQVNYTEWKFSETNSFIGDGFKNQHEEEEMTLSHSALKQKEPLHPVNDKESSEQGSEVSEAQTTDSDDVIVPPMSQKYPKADSEKMCIEIVSLAFYPEAEVMSDENIKQVYVEYKFYDLPLSETETPVSLRKPRAGEEIHFHFSKVIDLDPQEQQGRRRFLFDMLNGQDPDQGHLKFTVVSDPLDEEKKECEEVGYAYLQLWQILESGRDILEQELDIVSPEDLATPIGRLKVSLQAAAVLHAIYKEMTEDLFS Predict reactive species Full Name: retinitis pigmentosa GTPase regulator interacting protein 1 Calculated Molecular Weight: 147 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC039089 Gene Symbol: RPGRIP1 Gene ID (NCBI): 57096 RRID: AB_10639367 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96KN7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.