Proteintech, 13315-1-AP, SLD5 Polyclonal antibody

Size: 20ul / 150ul The SLD5 (13315-1-AP) by Proteintech is a Polyclonal antibody targeting SLD5 in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 13315-1-AP targets SLD5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse thymus tissue, mouse testis tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information SLD5 (DNA replication complex GINS protein SLD5) is also named as GINS4 (GINS complex subunit 4). SLD5 is required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks (PMID: 17417653). SLD5 forms a GINS complex with PSF1, PSF2 and PSF3, which is essential for the initiation of DNA replication in lower eukaryotes (PMID: 24244394). SLD5 is a member of the GINS complex, essential for DNA replication in eukaryotes. It has been reported that SLD5 is involved in early embryogenesis in the mouse, and cell cycle progression and genome integrity in Drosophila (PMID: 27499248). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4158 Product name: Recombinant human SLD5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-100 aa of BC027454 Sequence: MTEEVDFLGQDSDGGSEEVVLTPAELIERLEQAWMNEKFAPELLESKPEIVECVMEQLEHMEENLRRAKREDLKVSIHQMEMERIRYVLSSYLRCRLMKV Predict reactive species Full Name: GINS complex subunit 4 (Sld5 homolog) Calculated Molecular Weight: 223 aa, 26 kDa Observed Molecular Weight: 26 kDa GenBank Accession Number: BC027454 Gene Symbol: SLD5 Gene ID (NCBI): 84296 RRID: AB_2109671 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BRT9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.