Proteintech, 13375-1-AP, SCFD2 Polyclonal antibody

Size: 20ul / 150ul The SCFD2 (13375-1-AP) by Proteintech is a Polyclonal antibody targeting SCFD2 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 13375-1-AP targets SCFD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: COLO 320 cells, HepG2 cells, Jurkat cells, K-562 cells Positive IP detected in: COLO 320 cells Positive IHC detected in: human colon cancer tissue, human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4197 Product name: Recombinant human SCFD2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC032453 Sequence: MSASGVLSFTQQGWEQVLAKVKRAVVYLDAACAESLHWGCGSTRLLEAVGGPDCHLREFEPDAIGGGAKQPKAVFVLSCLLKGRTVEILRDIICRSHFQYCVVVTTVSHAVHLTANHVPAAAAAEMEGQQPVFEQLEEKLCEWMGNMNYTAEVFHVPLLLAPVAPHFALTPAFASLFPLLPQDVHLLNSARPDKRKLGSLGDVDSTTLTPELLLQIRCLVSGLSSLCEHLGVREECFAVGSLSQVIAADLANYAPAKNRKKTAAGRASVVFVDRTLDLTGAVGHHGDNLVEKIISALPQLPGHTNDVMVNMIALTALHTEEENYNVVAPGCLSQSSDTTAKALWEALLNTK Predict reactive species Full Name: sec1 family domain containing 2 Calculated Molecular Weight: 684 aa, 75 kDa Observed Molecular Weight: 70-75 kDa GenBank Accession Number: BC032453 Gene Symbol: SCFD2 Gene ID (NCBI): 152579 RRID: AB_10646473 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8WU76 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.