Proteintech, 13399-1-AP, Zinc Alpha 2 Glycoprotein Polyclonal antibody

Size: 20ul / 150ul The Zinc Alpha 2 Glycoprotein (13399-1-AP) by Proteintech is a Polyclonal antibody targeting Zinc Alpha 2 Glycoprotein in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 13399-1-AP targets Zinc Alpha 2 Glycoprotein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: human plasma, MCF-7 cells Positive IHC detected in: mouse liver tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, PC-3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4232 Product name: Recombinant human AZGP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-298 aa of BC033830 Sequence: MVRMVPVLLSLLLLLGPAVPQENQDGRYSLTYIYTGLSKHVEDVPAFQALGSLNDLQFFRYNSKDRKSQPMGLWRQVEGMEDWKQDSQLQKAREDIFMETLKDIVEYYNDSNGSHVLQGRFGCEIENNRSSGAFWKYYYDGKDYIEFNKEIPAWVPFDPAAQITKQKWEAEPVYVQRAKAYLEEECPATLRKYLKYSKNILDRQDPPSVVVTSHQAPGEKKKLKCLAYDFYPGKIDVHWTRAGEVQEPELRGDVLHNGNGTYQSWVVVAVPPQDTAPYSCHVQHSSLAQPLVVPWEAS Predict reactive species Full Name: alpha-2-glycoprotein 1, zinc-binding Calculated Molecular Weight: 298 aa, 34 kDa Observed Molecular Weight: 41 kDa GenBank Accession Number: BC033830 Gene Symbol: Zinc Alpha 2 Glycoprotein Gene ID (NCBI): 563 RRID: AB_2062582 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P25311 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.