Proteintech, 13426-1-AP, DLAT Polyclonal antibody

Size: 20ul / 150ul The DLAT (13426-1-AP) by Proteintech is a Polyclonal antibody targeting DLAT in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 13426-1-AP targets DLAT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, A431 cells, HAP1 cells, HEK-293 cells, HepG2 cells, Jurkat cells, LNCaP cells, MCF-7 cells Positive IP detected in: HepG2 cells Positive IHC detected in: human liver cancer tissue, human colon cancer tissue, mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, rabbit, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4226 Product name: Recombinant human DLAT protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-322 aa of BC039084 Sequence: MWRVCARRAQNVAPWAGLEARWTALQEVPGTPRVTSRSGPAPARRNSVTTGYGGVRALCGWTPSSGATPRNRLLLQLLGSPGRRYYSLPPHQKVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPT Predict reactive species Full Name: dihydrolipoamide S-acetyltransferase Calculated Molecular Weight: 647 aa, 70 kDa Observed Molecular Weight: 69-70 kDa GenBank Accession Number: BC039084 Gene Symbol: DLAT Gene ID (NCBI): 1737 RRID: AB_2091774 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P10515 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.