Proteintech, 13449-1-AP, PDGFR beta/CD140b Polyclonal antibody

Size: 20ul / 150ul The PDGFR beta/CD140b (13449-1-AP) by Proteintech is a Polyclonal antibody targeting PDGFR beta/CD140b in WB, IHC, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples 13449-1-AP targets PDGFR beta/CD140b in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse lung tissue Positive IP detected in: mouse lung tissue Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse colon tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information PDGFRs are receptor tyrosine kinases with an extracellular ligand-binding domain and a cytoplasmic enzymatic domain. The signaling activity of PDGFRs is tightly regulated by an autoinhibitory allosteric conformation, and requires ligand-induced receptor dimerization at the cell surface to alleviate autoinhibition and achieve physiological activity (PMID: 21664579). PDGFR beta (platelet-derived growth factor receptor- β)/CD140b is a constitutive marker expressed by ASCs in the angiogenic behavior of human retinal endothelial cells (HREs). CD140b (PDGFRβ) plays a crucial role in controlling the intrinsic proliferation (PMID: 30976657) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4285 Product name: Recombinant human PDGFRB protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 755-1106 aa of BC032224 Sequence: LDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICEGKLVKICDFGLARDIMRDSNYISKGSTFLPLKWMAPESIFNSLYTTLSDVWSFGILLWEIFTLGGTPYPELPMNEQFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFEIRPPFSQLVLLLERLLGEGYKKKYQQVDEEFLRSDHPAILRSQARLPGFHGLRSPLDTSSVLYTAVQPNEGDNDYIIPLPDPKPEVADEGPLEGSPSLASSTLNEVNTSSTISCDSPLEPQDEPEPEPQLELQVEPEPELEQLPDSGCPAPRAEAEDSFL Predict reactive species Full Name: platelet-derived growth factor receptor, beta polypeptide Calculated Molecular Weight: 1106 aa, 124 kDa Observed Molecular Weight: 160-180 kDa GenBank Accession Number: BC032224 Gene Symbol: PDGFR beta Gene ID (NCBI): 5159 RRID: AB_2162644 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P09619 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.