Proteintech, 13491-1-AP, EGR2 Polyclonal antibody

Size: 20ul / 150ul The EGR2 (13491-1-AP) by Proteintech is a Polyclonal antibody targeting EGR2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 13491-1-AP targets EGR2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Daudi cells, mouse brain tissue, mouse kidney tissue, SH-SY5Y cells, MCF-7 cells, PC-3 cells, HepG2 cells, rat brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information EGR2 is also named as KROX20, belongs to the EGR C2H2-type zinc-finger protein family. As a zinc finger transcription factor, it is observed in both the somata and dendrites of central neurons(PMID: 12706208). EGR2 plays an important role in the transient formation of hindbrain developmental compartments or rhombomeres and is also an important factor in peripheral myelination, maintenance of synaptic plasticity and long-term potentiation(PMID: 7707882, 8619872, 8895453, 16203212, 18280047). Egr2 expression is induced downstream of TCR signaling in NKT precursors and Egr2 is directly connected with the key molecular checkpoints defining NKT lineage commitment and stage progression, suggesting that Egr2 not only induces the early lineage-defining transcription factor PLZF, but also controls the downstream expression of the IL-2Rβ chain(PMID: 22306690). Egr2 is regulated by both soluble and membrane-bound neuregulins(PMID: 16129398, 18803322, 8787758) and its concentration is partially modulated by calcium-dependent events(PMID: 19179536). Egr2/Krox20 is also required for induction of Pmp22 (PMID: 21411665), and is nuclear-localized(PMID: 12706208). On western blotting, the observed brand is around 50 kDa or gives a raise around 63 kDa(PMID: 22792185). This antibody is a rabbit polyclonal antibody raised against a region of human EGR2. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4313 Product name: Recombinant human EGR2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 150-499 aa of BC035625 Sequence: GVCTMSQTQPDLDHLYSPPPPPPPYSGCAGDLYQDPSAFLSAATTSTSSSLAYPPPPSYPSPKPATDPGLFPMIPDYPGFFPSQCQRDLHGTAGPDRKPFPCPLDTLRVPPPLTPLSTIRKPFPCPLDTLRVPPPLTPLSTIRNFTLGGPSAGVTGPGASGGSEGPRLPGSSSAAAAAAAAAAYNPHHLPLRPILRPRKYPNRPSKTPVHERPYPCPAEGCDRRFSRSDELTRHIRIHTGHKPFQCRICMRNFSRSDHLTTHIRTHTGEKPFACDYCGRKFARSDERKRHTKIHLRQKERKSSAPSASVPAPSTASCSGGVQPGGTLCSSNSSSLGGGPLAPCSSRTRTP Predict reactive species Full Name: early growth response 2 (Krox-20 homolog, Drosophila) Calculated Molecular Weight: 476 aa, 50 kDa Observed Molecular Weight: 60-70 kDa GenBank Accession Number: BC035625 Gene Symbol: EGR2 Gene ID (NCBI): 1959 RRID: AB_2097177 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P11161 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.