Proteintech, 13493-1-AP, GP2 Polyclonal antibody

Size: 20ul / 150ul The GP2 (13493-1-AP) by Proteintech is a Polyclonal antibody targeting GP2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 13493-1-AP targets GP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat pancreas tissue, rat brain tissue, mouse pancreas tissue Positive IHC detected in: mouse pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Pancreatic secretory granule membrane major glycoprotein (GP2) is the most abundant membrane component of zymogen granules secreted by pancreatic acinar cells (PMID: 8190715). GP2 associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. It is released from the membrane of mature zymogen granules by an enzymatic mechanism (PMID: 2011597). GP2 plays a role in defense against adhesive and invasive commensal bacteria during intestinal inflammation (PMID: 33594081). GP2 is preferentially expressed in acinar cell carcinomas of the pancreas but the glycoprotein can rarely also be expressed in a variety of other tumor entities (PMID: 36137400). The apparent molecular weight of GP2 is 70-100 kDa, which is larger than the calculated molecular weight, due to glycosylation (PMID: 19627615; 30186250). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4317 Product name: Recombinant human GP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-360 aa of BC032693 Sequence: MERMVGSGLLWLALVSCILTQASAVQRDPSTVEDKCEKACRPEEECLALNSTWGCFCRQDLNSSDVHSLQPQLDCGPREIKVKVDKCLLGGLGLGEEVIAYLRDPNCSSILQTEERNWVSVTSPVQASACRNILERNQTHAIYKNTLSLVNDFIIRDTILNINFQCAYPLDMKVSLQAALQPIVSSLNVSVDGNGEFIVRMALFQDQNYTNPYQGDAVELSVESVLYVGAILEQGDTSRFNLVLRNCYATPTEDKADLVKYFIIRNSCSNQRDSTIHVEENGQSSESRFSVQMFMFAGHYDLVFLHCEIHLCDSLNEQCQPSCSRSQVRSEVPAIDLARVLDLGPITRRGAQSPGVMNGT Predict reactive species Full Name: glycoprotein 2 (zymogen granule membrane) Calculated Molecular Weight: 530 aa, 58 kDa Observed Molecular Weight: 75-85 kDa GenBank Accession Number: BC032693 Gene Symbol: GP2 Gene ID (NCBI): 2813 RRID: AB_3669164 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P55259 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.