Proteintech, 13520-1-AP, Choline Kinase Alpha Polyclonal antibody

Size: 20ul / 150ul The Choline Kinase Alpha (13520-1-AP) by Proteintech is a Polyclonal antibody targeting Choline Kinase Alpha in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 13520-1-AP targets Choline Kinase Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: COLO 320 cells, mouse colon tissue, mouse spleen tissue, rat spleen tissue Positive IP detected in: mouse spleen tissue Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CHKA(Choline kinase alpha) is also named as CHK, CKI, EK(Ethanolamine kinase) and belongs to the choline/ethanolamine kinase family. Choline kinase (ATP:choline phosphotransferase) is a cytosolic enzyme that catalyzes the committed step in the synthesis of PC by the CDP-choline pathway. The enzyme catalyzes the phosphorylation of choline with ATP to form phosphocholine and ADP(PMID:9506987). It has 2 isoforms produced by alternative splicing with the molecular weight of 52 kDa and 50 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4761 Product name: Recombinant human CHKA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 139-439 aa of BC036471 Sequence: EPRKVLLRLYGAILQVGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELGLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGQWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV Predict reactive species Full Name: choline kinase alpha Calculated Molecular Weight: 439 aa, 52 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC036471 Gene Symbol: CHKA Gene ID (NCBI): 1119 RRID: AB_2079160 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P35790 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.