Proteintech, 13554-1-AP, HELIOS Polyclonal antibody

Size: 20ul / 150ul The HELIOS (13554-1-AP) by Proteintech is a Polyclonal antibody targeting HELIOS in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 13554-1-AP targets HELIOS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, human placenta tissue Positive IP detected in: Jurkat cells Positive IHC detected in: human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Ikaros DNA-binding proteins, characterized by two highly conserved zinc finger domains that mediate DNA (N-terminal domain) and protein binding (C-terminal domain), consists of 5 members and one of them is IKZF2 [PMID:1439790]. IKZF2's expression is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4357 Product name: Recombinant human IKZF2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 227-526 aa of BC028936 Sequence: NNISLVPFERPAVIEKLTGNMGKRKSSTPQKFVGEKLMRFSYPDIHFDMNLTYEKEAELMQSHMMDQAINNAITYLGAEALHPLMQHPPSTIAEVAPVISSAYSQVYHPNRIERPISRETADSHENNMDGPISLIRPKSRPQEREASPSNSCLDSTDSESSHDDHQSYQGHPALNPKRKQSPAYMKEDVKALDTTKAPKGSLKDIYKVFNGEGEQIRAFKCEHCRVLFLDHVMYTIHMGCHGYRDPLECNICGYRSQDRYEFSSHIVRGEHTFH Predict reactive species Full Name: IKAROS family zinc finger 2 (Helios) Calculated Molecular Weight: 500 aa, 57 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC028936 Gene Symbol: HELIOS Gene ID (NCBI): 22807 RRID: AB_2124959 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UKS7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.