Proteintech, 13562-1-AP, CYP2J2 Polyclonal antibody

Size: 20ul / 150ul The CYP2J2 (13562-1-AP) by Proteintech is a Polyclonal antibody targeting CYP2J2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 13562-1-AP targets CYP2J2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HUVEC cells, L02 cells, mouse skeletal muscle tissue, mouse heart tissue, HepG2 cells, rat heart tissue Positive IHC detected in: human liver tissue, mouse heart tissue, human liver cancer tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information CYP2J2(Cytochrome P450 2J2) belongs to the cytochrome P450 family. This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. The human CYP2J member, CYP2J2, is shown to be expressed in several tissues such as the small intestine, liver, heart, kidney, and lung and may play a role in the oxidative bioactivation of arachidonic acid(PMID:12386130). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4435 Product name: Recombinant human CYP2J2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 152-502 aa of BC032594 Sequence: KKSLEERIQEEAQHLTEAIKEENGQPFDPHFKINNAVSNIICSITFGERFEYQDSWFQQLLKLLDEVTYLEASKTCQLYNVFPWIMKFLPGPHQTLFSNWKKLKLFVSHMIDKHRKDWNPAETRDFIDAYLKEMSKHTGNPTSSFHEENLICSTLDLFFAGTETTSTTLRWALLYMALYPEIQEKVQAEIDRVIGQGQQPSTAARESMPYTNAVIHEVQRMGNIIPLNVPREVTVDTTLAGYHLPKGTMILTNLTALHRDPTEWATPDTFNPDHFLENGQFKKREAFMPFSIGKRACLGEQLARTELFIFFTSLMQKFTFRPPNNEKLSLKFRMGITISPVSHRLCAVPQV Predict reactive species Full Name: cytochrome P450, family 2, subfamily J, polypeptide 2 Calculated Molecular Weight: 502 aa, 58 kDa Observed Molecular Weight: 58-65 kDa GenBank Accession Number: BC032594 Gene Symbol: CYP2J2 Gene ID (NCBI): 1573 RRID: AB_2090013 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P51589 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.