Proteintech, 13585-1-AP, PRDX6 Polyclonal antibody

Size: 20ul / 150ul The PRDX6 (13585-1-AP) by Proteintech is a Polyclonal antibody targeting PRDX6 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 13585-1-AP targets PRDX6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: SH-SY5Y cells, A549 cells, HeLa cells, HAP1, K-562 cells, HepG2 cells, mouse liver tissue, rat liver tissue Positive IP detected in: HeLa cells, HAP1 cells Positive IHC detected in: human liver tissue, human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:100-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4512 Product name: Recombinant human PRDX6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-224 aa of BC035857 Sequence: MPGGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPATTGRNFDEILRVVISLQLTAEKRVATPVDWKDGDSVMVLPTIPEEEAKKLFPKGVFTKELPSGKKYLRYTPQP Predict reactive species Full Name: peroxiredoxin 6 Calculated Molecular Weight: 224 aa, 25 kDa Observed Molecular Weight: 25-30 kDa GenBank Accession Number: BC035857 Gene Symbol: PRDX6 Gene ID (NCBI): 9588 RRID: AB_2168637 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P30041 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.