Proteintech, 13634-1-AP, TROAP Polyclonal antibody

Size: 20ul / 150ul The TROAP (13634-1-AP) by Proteintech is a Polyclonal antibody targeting TROAP in WB, IHC, ELISA applications with reactivity to human, mouse samples 13634-1-AP targets TROAP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, HCT 116 cells, PC-3 cells, HT-29 cells, Jurkat cells Positive IHC detected in: human gliomas tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Background Information TROAP, also known as tastin, was first cloned from human epithelial cells in 1995. It as a cytoplasmic protein is composed of 778 amino acid residues and contains potential phosphorylation sites for protein kinases. TROAP highly expresses in bone marrow, testis and thymus, and is involved in centrosome integrity and spindle assembly during mitosis (PMID: 33500384). TROAP has been shown to promote some tumor progressions, such as those of breast and colorectal cancers (PMID: 33692939). Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4562 Product name: Recombinant human TROAP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 428-778 aa of BC032583 Sequence: QEVKIQRIGILQQLLRQEVEGLVGGQCVPLNGGSSLDMVELQPLLTEISRTLNATEHNSGTSHLPGLLKHSGLPKPCLPEECGEPQPCPPAEPGPPEAFCRSEPEIPEPSLQEQLEVPEPYPPAEPRPLESCCRSEPEIPESSRQEQLEVPEPCPPAEPRPLESYCRIEPEIPESSRQEQLEVPEPCPPAEPGPLQPSTQGQSGPPGPCPRVELGASEPCTLEHRSLESSLPPCCSQWAPATTSLIFSSQHPLCASPPICSLQSLRPPAGQAGLSNLAPRTLALRERLKSCLTAIHCFHEARLDDECAFYTSRAPPSGPTRVCTNPVATLLEWQDALCFIPVGSAAPQGSP Predict reactive species Full Name: trophinin associated protein (tastin) Calculated Molecular Weight: 778 aa, 84 kDa Observed Molecular Weight: 110 kDa GenBank Accession Number: BC032583 Gene Symbol: TROAP Gene ID (NCBI): 10024 RRID: AB_10597857 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q12815 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.