Proteintech, 13700-1-AP, TBX21/T-bet Polyclonal antibody

Size: 20ul / 150ul The TBX21/T-bet (13700-1-AP) by Proteintech is a Polyclonal antibody targeting TBX21/T-bet in WB, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse samples 13700-1-AP targets TBX21/T-bet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NK-92 cells, A549 cells, mouse thymus tissue, RAW 264.7 cells Positive IP detected in: mouse thymus tissue Positive IF/ICC detected in: NK-92 cells Positive FC (Intra) detected in: NK92 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4618 Product name: Recombinant human TBX21,T-bet protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC039739 Sequence: MGIVEPGCGDMLTGTEPMPGSDEGRAPGADPQHRYFYPEPGAQDADERRGGGSLGSPYPGGALVPAPPSRFLGAYAYPPRPQAAGFPGAGESFPPPADAEGYQPGEGYAAPDPRAGLYPGPREDYALPAGLEVSGKLRVALNNHLLWSKFNQHQTEMIITKQGRRMFPFLSFTVAGLEPTSHYRMFVDVVLVDQHHWRYQSGKWVQCGKAEGSMPGNRLYVHPDSPNTGAHWMRQEVSFGKLKLTNNKGASNNVTQMIVLQSLHKYQPRLHIVEVNDGEPEAACNASNTHIFTFQETQFIAVTAYQNAEITQLKIDNNPFAKGFRENF Predict reactive species Full Name: T-box 21 Calculated Molecular Weight: 535 aa, 58 kDa Observed Molecular Weight: 60-62 kDa GenBank Accession Number: BC039739 Gene Symbol: TBX21/T-bet Gene ID (NCBI): 30009 ENSEMBL Gene ID: ENSG00000073861 RRID: AB_10666436 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UL17 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.