Proteintech, 13779-1-AP, DZIP1 Polyclonal antibody

Size: 20ul / 150ul The DZIP1 (13779-1-AP) by Proteintech is a Polyclonal antibody targeting DZIP1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 13779-1-AP targets DZIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, human brain tissue, human testis tissue, mouse skeletal muscle tissue, PC-3 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human oesophagus cancer tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: PC-3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information DZIP1, also named as DZIP, DZIP2 and KIAA0996, belongs to the DZIP C2H2-type zinc-finger protein family. DZIP1 has been reported to be associated with centrosomes and ciliogenesis in cells (PMID: 32051257). DZIP1 may participate in spermatogenesis via its interaction with DAZ1, and play a role in primary formation (PMID: 15081113, 19852954). DZIP1 has 3 isoforms with the molecular mass of 31, 97 and 99 kDa. It may be a potential therapeutic target of oral squamous carcinoma (PMID: 31450126). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4772 Product name: Recombinant human DZIP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 496-867 aa of BC041804 Sequence: FSSHILEPIEELSEEEKGRENEQKLNNNKMHLRKALKSNSSLTKGLRTMVEQNLMEKLETLGINADIRGISSDQLHRVLKSVESERHKQEREIPNFHQIREFLEHQVSCKIEEKALLSSDQCSVSQMDTLSTGEVPKMIQLPSKNRQLIRQKAVSTDRTSVPKIKKNVMEDPFPRKSSTITTPPFSSEEEQEDDDLIRAYASPGPLPVPPPQNKGSFGKNTVKSDADGTEGSEIEDTDDSPKPAGVAVKTPTEKVEKMFPHRKNVNKPVGGTNVPEMFIKKEELQELKCADVEDEDWDISSLEEEISLGKKSGKEQKEPPPAKNEPHFAHVLNAWGAFNPKGPKGEGLQENESSTLKSSLVTVTDWSDTSDV Predict reactive species Full Name: DAZ interacting protein 1 Calculated Molecular Weight: 867 aa, 98 kDa Observed Molecular Weight: 100-113 kDa GenBank Accession Number: BC041804 Gene Symbol: DZIP1 Gene ID (NCBI): 22873 RRID: AB_2096927 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86YF9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.