Proteintech, 13812-1-AP, NPHP4 Polyclonal antibody

Size: 20ul / 150ul The NPHP4 (13812-1-AP) by Proteintech is a Polyclonal antibody targeting NPHP4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, canine samples 13812-1-AP targets NPHP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples. Tested Applications Positive WB detected in: mouse brain tissue, Y79 cells Positive IHC detected in: human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: hTERT-RPE1 cells, MDCK cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information NPHP4, also named as KIAA0673, is a cilia-associated protein which involved in the organization of apical junctions in kidney cells together with NPHP1 and RPGRIP1L/NPHP8. It is necessary for normal photoreceptor ribbon synapse maintenance and outer segment formation, and for sperm development. Specification Tested Reactivity: human, mouse, canine Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4875 Product name: Recombinant human NPHP4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC040520 Sequence: MNDWHRIFTQNVLVPPHPQRARQPWKESTAFQCVLKWLDGPVIRQGVLEVLSEVECHLRVSFFDVTYRHFFGRTWKTTVKPTKRPPSRIVFNEPLYFHTSLNHPHIVAVVEVVAEGKKRDGSLQTLSCGFGILRIFSNQPDSPISASQDKRLRLYHGTPRALLHPLLQDPAEQNRHMTLIENCSLQYTLKPHPALEPAFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPITGHLDDLFFTLYPSLEKFEEELLELHVQDHFQEGCGPLDGGALEILERRLRVGVHNGLGFVQRPQVVVLVPEMDVALTRSASFSRKVVSSSKTSSGSQALVLRSRLRLPEMVG Predict reactive species Full Name: nephronophthisis 4 Calculated Molecular Weight: 1426 aa, 158 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: BC040520 Gene Symbol: NPHP4 Gene ID (NCBI): 261734 RRID: AB_10640302 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75161 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.