Proteintech, 13843-1-AP, CNTN1 Polyclonal antibody

Size: 20ul / 150ul The CNTN1 (13843-1-AP) by Proteintech is a Polyclonal antibody targeting CNTN1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 13843-1-AP targets CNTN1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, A549 cells, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue Positive IP detected in: rat brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Contactin-1 (CNTN1), also known as Glycoprotein gp135 or Neural cell surface protein F3, is a neuronal cell adhesion molecule of the immunoglobulin superfamily (PMID: 3049624). It consists of six Ig domains, four fibronectin-like motifs, and a glycosylphosphatidylinositol (GPI)-moiety. Expressed by the central and peripheral neurons, CNTN1 contributes to the formation and function of neuronal connections. It plays a role in axon extension and formation of septate-like junctions between axons and myelinating glial cells (PMID: 23724143). In addition, acting as a ligand for Notch, CNTN1 initiates a NOTCH/DTX1 signaling pathway that promotes oligodendrocyte maturation and myelination (PMID: 14567914). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4803 Product name: Recombinant human CNTN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 26-351 aa of BC036569 Sequence: RRYGHGVSEEDKGFGPIFEEQPINTIYPEESLEGKVSLNCRARASPFPVYKWRMNNGDVDLTSDRYSMVGGNLVINNPDKQKDAGIYYCLASNNYGMVRSTEATLSFGYLDPFPPEERPEVRVKEGKGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVEASDKGNYSCFVSSPSITKSVFSKFIPLIPIPERTTKPYPADIVVQFKDVYALMGQNVTLECFALGNPVPDIRWRKVLEPMPSTAEISTSGAVLKIFNIQLEDEGIYECEAENIRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYWP Predict reactive species Full Name: contactin 1 Calculated Molecular Weight: 1018 aa, 113 kDa Observed Molecular Weight: 135 kDa GenBank Accession Number: BC036569 Gene Symbol: CNTN1 Gene ID (NCBI): 1272 RRID: AB_2877981 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q12860 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.