Proteintech, 13855-1-AP, FBX4 Polyclonal antibody

Size: 20ul / 150ul The FBX4 (13855-1-AP) by Proteintech is a Polyclonal antibody targeting FBX4 in IP, ELISA applications with reactivity to human samples 13855-1-AP targets FBX4 in IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive IP detected in: HeLa cells, A431 cells Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information FBX4 (F-Box Protein 4), also known as FBXO4, is a member of the F-box protein family. FBX4 is involved in the ubiquitination of various target proteins, leading to their degradation. It has been shown to regulate the cell cycle by targeting proteins such as cyclin D1 for ubiquitination and degradation. Mutations in FBX4 have been linked to various diseases, including spermatogenic failure and esophageal cancer. In cancer, FBX4 has been shown to act as a tumor suppressor. Loss of FBX4 function can lead to increased cell proliferation and tumor development. For example, FBX4 knockout mice have been shown to develop various tumors, including lymphoblastic lymphoma and mammary carcinoma. FBX4 has two isforms: 44 kDa and 35 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4829 Product name: Recombinant human FBX4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-387 aa of BC048098 Sequence: MAGSEPRSGTNSPPPPFSDWGRLEAAILSGWKTFWQSVSKERVARTTSREEVDEAASTLTRLPIDVQLYILSFLSPHDLCQLGSTNHYWNETVRDPILWRYFLLRDLPSWSSVDWKSLPDLEILKKPISEVTDGAFFDYMAVYRMCCPYTRRASKSSRPMYGAVTSFLHSLIIQNEPRFAMFGPGLEELNTSLVLSLMSSEELCPTAGLPQRQIDGIGSGVNFQLNNQHKFNILILYSTTRKERDRAREEHTSAVNKMFSRHNEGDDQQGSRYSVIPQIQKVCEVVDGFIYVANAEAHKRHEWQDEFSHIMAMTDPAFGSSGRPLLVLSCISQGDVKRMPCFYLAHELHLNLLNHPWLVQDTEAETLTGFLNGIEWILEEVESKRAR Predict reactive species Full Name: F-box protein 4 Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 38 kDa GenBank Accession Number: BC048098 Gene Symbol: FBXO4/FBX4 Gene ID (NCBI): 26272 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UKT5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.