Proteintech, 13873-1-AP, DGKH Polyclonal antibody

Size: 20ul / 150ul The DGKH (13873-1-AP) by Proteintech is a Polyclonal antibody targeting DGKH in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 13873-1-AP targets DGKH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information DGKH(Diacylglycerol kinase eta) is also named as DGK-eta and belongs to the eukaryotic diacylglycerol kinase family. It is a key protein in the lithium-sensitive phosphatidyl inositol pathway, and recently a link between environmental stress and schizophrenia/bipolar disorder has been revealed. DGKH has some isoforms produced by alternative splicing. Two isoforms DGKH1 and DGKH2 with the molecular weight of 128 kDa and 135 kDa shows diacylglycerol kinase activity; however, the 2 isoforms differed in several other characteristics(PMID:12810723). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4926 Product name: Recombinant human DGKH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 754-1100 aa of BC043292 Sequence: MAVSRVIKLQHHRIAQCRTVKITIFGDEGVPVQVDGEAWVQPPGIIKIVHKNRAQMLTRDRAFESTLKSWEDKQKCDSGKPVLRTHLYIHHAIDLATEEVSQMQLCSQAAEELITRICDAATIHCLLEQELAHAVNACSHALNKANPRCPESLTRDTATEIAINVKALYNETESLLVGRVPLQLESPHEERVSNALHSVEVELQKLTEIPWLYYILHPNEDEEPPMDCTKRNNRSTVFRIVPKFKKEKVQKQKTSSQPVQKWGTEEVAAWLDLLNLGEYKDIFIRHDIRGAELLHLERRDLKNTVGEKRDTKENGKHMDLGIPKVGHVKRILQGIKELGRSTPQSEV Predict reactive species Full Name: diacylglycerol kinase, eta Calculated Molecular Weight: 1100 aa, 122 kDa Observed Molecular Weight: 135 kDa GenBank Accession Number: BC043292 Gene Symbol: DGKH Gene ID (NCBI): 160851 RRID: AB_2091287 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86XP1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.