Proteintech, 13889-1-AP, DHH Polyclonal antibody

Size: 20ul / 150ul The DHH (13889-1-AP) by Proteintech is a Polyclonal antibody targeting DHH in WB, ELISA applications with reactivity to human, mouse, rat samples 13889-1-AP targets DHH in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse skeletal muscle tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Desert hedgehog (DHH) is one of three ligands in the hedgehog signaling pathway that controls gonadmorphogenesis and nerve perineurium differentiation.What is the molecular weight of DHH? Is DHH post-translationally modified?DHH protein is synthesized in a precursor form of ~45 kDa. Hedgehog proteins contain asmall N-terminal signalpeptide, which iscleaved off intracellularly, leading to 39 kDa species. Hedgehog proteins are then subject toautoproteolysis, which leads to the generation of a 19 kDa N-terminal fragment with added cholesterol moietyand a 25 kDa C-terminal fragment (PMID: 7985023). The C-terminal fragment catalyzes the precursor proteolysis,while theN-terminal fragment gets secreted from cells and acts as an active ligand in the hedgehog signalingpathway.What is the subcellular localization of DHH?DHH protein is subject to autoproteolysis, as described above, and the N-terminal fragment is secreted fromcells in a regulated manner. The amounts of extracellular hedgehog proteins determine local and long-rangesignaling (PMID: 7736596 and 7885476).What is the tissue expression pattern of DHH?Unlike other hedgehog ligands, expression of DHH is very tissue-specific. DHH is strongly expressed in Sterolicellprecursors,adult sterol cells, and in Leydig cells in testis (PMID: 8805249, 12050120)but also ingranulosacells in ovaries (PMID: 15878962), as well as in Schwann cells-glia of the peripheral nervous system(PMID: 7589793).What is the role of DHH in morphogenesis?DHH plays a role in the correct development of male gonads and perineurium. Loss of DHH leads to peripheralneuropathy and gonadal dysgenesis (PMID: 11017805). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4850 Product name: Recombinant human DHH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 51-396 aa of BC033507 Sequence: GVPERTLGASGPAEGRVARGSERFRDLVPNYNPDIIFKDEENSGADRLMTERCKERVNALAIAVMNMWPGVRLRVTEGWDEDGHHAQDSLHYEGRALDITTSDRDRNKYGLLARLAVEAGFDWVYYESRNHVHVSVKADNSLAVRAGGCFPGNATVRLWSGERKGLRELHRGDWVLAADASGRVVPTPVLLFLDRDLQRRASFVAVETEWPPRKLLLTPWHLVFAARGPAPAPGDFAPVFARRLRAGDSVLAPGGDALRPARVARVAREEAVGVFAPLTAHGTLLVNDVLASCYAVLESHQWAHRAFAPLRLLHALGALLPGGAVQPTGMHWYSRLLYRLAEELLG Predict reactive species Full Name: desert hedgehog homolog (Drosophila) Calculated Molecular Weight: 396 aa, 44 kDa Observed Molecular Weight: 42 kDa GenBank Accession Number: BC033507 Gene Symbol: DHH Gene ID (NCBI): 50846 RRID: AB_2814660 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O43323 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.