Proteintech, 13897-1-AP, GTPBP4 Polyclonal antibody

Size: 20ul / 150ul The GTPBP4 (13897-1-AP) by Proteintech is a Polyclonal antibody targeting GTPBP4 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 13897-1-AP targets GTPBP4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, mouse testis tissue, human kidney tissue, HepG2 cells Positive IP detected in: HeLa cells, mouse testis tissue Positive IF/ICC detected in: MDCK cells Recommended dilution Western Blot (WB): WB : 1:2000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, goat, drosophila Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4859 Product name: Recombinant human GTPBP4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 332-634 aa of BC038975 Sequence: KTEACDRLLAHRVETKMKGNKVNEVLNRLHLAIPTRRDDKERPPFIPEGVVARRKRMETEESRKKRERDLELEMGDDYILDLQKYWDLMNLSEKHDKIPEIWEGHNIADYIDPAIMKKLEELEKEEELRTAAGEYDSVSESEDEEMLEIRQLAKQIREKKKLKILESKEKNTQGPRMPRTAKKVQRTVLEKEMRSLGVDMDDKDDAHYAVQARRSRSITRKRKREDSAPPSSVARSGSCSRTPRDVSGLRDVKMVKKAKTMMKNAQKKMNRLGKKGEADRHVFDMKPKHLLSGKRKAGKKDRR Predict reactive species Full Name: GTP binding protein 4 Calculated Molecular Weight: 634 aa, 74 kDa Observed Molecular Weight: 74 kDa GenBank Accession Number: BC038975 Gene Symbol: GTPBP4 Gene ID (NCBI): 23560 RRID: AB_2279486 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BZE4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.