Proteintech, 13903-1-AP, ZHX1 Polyclonal antibody

Size: 20ul / 150ul The ZHX1 (13903-1-AP) by Proteintech is a Polyclonal antibody targeting ZHX1 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 13903-1-AP targets ZHX1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, mouse heart tissue, mouse kidney tissue, Jurkat cells Positive IP detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Binding of the nuclear factor-Y complex (NF-Y) to the inverted CCAAT-box interferes with transcription activation through nucleosome reorganization. The three homologous proteins forming the zinc-fingers and homeoboxes (ZHX) family interact with the activation domain of NF-Ya to repress transcription [PMID:19348505,20509910]. Human zinc-fingers and homeoboxes 1 (ZHX1) was cloned as a protein that interacts with the activation domain (AD) of the A subunit of nuclear factor-Y (NF-YA) [PMID:12237128]. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4874 Product name: Recombinant human ZHX1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 524-873 aa of BC040481 Sequence: SNQCLHLNNDSSTTIIIDSSDETTESPTVGTAQPKQSWNPFPDFTPQKFKEKTAEQLRVLQASFLNSSVLTDEELNRLRAQTKLTRREIDAWFTEKKKSKALKEEKMEIDESNAGSSKEEAGETSPADESGAPKSGSTGKICKKTPEQLHMLKSAFVRTQWPSPEEYDKLAKESGLARTDIVSWFGDTRYAWKNGNLKWYYYYQSANSSSMNGLSSLRKRGRGRPKGRGRGRPRGRPRGSKRINNWDRGPSLIKFKTGTAILKDYYLKRKFLNEQDLDELVNKSHMGYEQVREWFAERQRRSELGIELFEENEEEDEVIDDQEEDEEETDDSDTWEPPRHVKRKLSKSDD Predict reactive species Full Name: zinc fingers and homeoboxes 1 Calculated Molecular Weight: 873 aa, 98 kDa Observed Molecular Weight: 100-120 kDa GenBank Accession Number: BC040481 Gene Symbol: ZHX1 Gene ID (NCBI): 11244 RRID: AB_2219214 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UKY1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.