Proteintech, 13949-1-AP, MAPKAPK2 Polyclonal antibody

Size: 20ul / 150ul The MAPKAPK2 (13949-1-AP) by Proteintech is a Polyclonal antibody targeting MAPKAPK2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 13949-1-AP targets MAPKAPK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, A549 cells, mouse skeletal muscle tissue, mouse colon tissue Positive IHC detected in: human breast cancer tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information MAPKAPK2(mitogen-activated protein kinase-activated protein kinase 2) is also named as MK2, MAPKAP-K2, MK-2 and belongs to the CAMK Ser/Thr protein kinase family. MAPKAPK2, one of several kinases directly phosphorylated and activated by p38 MAPK, plays a central role in the inflammatory response and is in the nucleus of unstimulated cells and moves rapidly to the cytoplasm after stimulation(PMID:12171911). It is also involved in many other cellular processes including stress responses, nuclear export, gene expression regulation and cell proliferation. Multiple residues of MAPKAPK2 are generally phosphorylated in vivo in response to stress, but only 4 residues(Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in vitro(PMID:22351694). It has 2 isoforms produced by alternative splicing and the range of the molecular weight is 42-60 kDa according to the references(PMID:10666409; 11328854;8995385). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5060 Product name: Recombinant human MAPKAPK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC036060 Sequence: MLSNSQGQSPPVPFPAPAPPPQPPTPALPHPPAQPPPPPPQQFPQFHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSLTTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVKEEMTSALATMRVDYEQIKIKKIEDASNPLLLKRRKKARALEAAALAH Predict reactive species Full Name: mitogen-activated protein kinase-activated protein kinase 2 Calculated Molecular Weight: 400 aa, 46 kDa Observed Molecular Weight: 47-50 kDa GenBank Accession Number: BC036060 Gene Symbol: MAPKAPK2 Gene ID (NCBI): 9261 RRID: AB_2877995 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P49137 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.