Proteintech, 13956-1-AP, PDE8A Polyclonal antibody

Size: 20ul / 150ul The PDE8A (13956-1-AP) by Proteintech is a Polyclonal antibody targeting PDE8A in WB, IP, IHC, ELISA applications with reactivity to human, mouse samples 13956-1-AP targets PDE8A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse testis tissue, mouse spleen tissue, SH-SY5Y cells Positive IP detected in: mouse testis tissue Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information PDE8A(high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A) belongs to the cyclic nucleotide phosphodiesterase family and PDE8 subfamily. PDE8A plays a critical role in the modulation of at least one compartment of cAMP and hence PKA activity during β-adrenergic receptor (βAR) activation in ventricular myocytes(PMID:20353794). The full length protein contains several sites for protein phosphorylation, N-glycosylation, N-myristoylation, and amidation. It has 5 isoforms produced by alternative splicing with the molecular mass of 93 kDa, 88 kDa, 51 kDa and the isoform 4 and 5 have the same molecular mass of 66 kDa.(PMID:11738832). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5069 Product name: Recombinant human PDE8A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 530-829 aa of BC060762 Sequence: FLHCSESTLRSWLQIIEANYHSSNPYHNSTHSADVLHATAYFLSKERIKETLDPIDEVAALIAATIHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTTGDDKCNIFKNMERNDYRTLRQGIIDMVLATEMTKHFEHVNKFVNSINKPLATLEENGETDKNQEVINTMLRTPENRTLIKRMLIKCADVSNPCRPLQYCIEWAARISEEYFSQTDEEKQQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFVDLPDLMQHLDNNFKYWKGLDEMKLRNLRPPPE Predict reactive species Full Name: phosphodiesterase 8A Calculated Molecular Weight: 93 kDa Observed Molecular Weight: 93 kDa, 88 kDa GenBank Accession Number: BC060762 Gene Symbol: PDE8A Gene ID (NCBI): 5151 RRID: AB_2161728 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60658 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.