Proteintech, 13960-1-AP, WDFY1 Polyclonal antibody

Size: 20ul / 150ul The WDFY1 (13960-1-AP) by Proteintech is a Polyclonal antibody targeting WDFY1 in WB, ELISA applications with reactivity to human, mouse, rat samples 13960-1-AP targets WDFY1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: THP-1 cells, mouse testis tissue, rat testis tissue Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Background Information WD repeating domain FYVE1 (WDFY1) belongs to the WD40 protein family, which plays key roles in diverse cellular processes, including signal transduction, gene transcription, vesicle fusion, and spermatogenesis(PMID: 32867693; PMID: 10322433). WDFY1 is highly expressed in mouse testis and is dispensable for mouse spermatogenesis and male fertility (PMID: 35121371). The bands of 24 kDa and 19 kDa may be potential isoforms of WDFY1. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4877 Product name: Recombinant human WDFY1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-410 aa of BC040525 Sequence: MAAEIHSRPQSSRPVLLSKIEGHQDAVTAALLIPKEDGVITASEDRTIRVWLKRDSGQYWPSIYHTMASPCSAMAYHHDSRRIFVGQDNGAVMEFHVSEDFNKMNFIKTYPAHQNRVSAIIFSLATEWVISTGHDKCVSWMCTRSGNMLGRHFFTSWASCLQYDFDTQYAFVGDYSGQITLLKLEQNTCSVITTLKGHEGSVACLWWDPIQRLLFSGASDNSIIMWDIGGRKGRTLLLQGHHDKVQSLCYLQLTRQLVSCSSDGGIAVWNMDVSREEAPQWLESDSCQKCEQPFFWNIKQMWDTKTLGLRQHHCRKCGQAVCGKCSSKRSSYPVMGFEFQVRVCDSCYDSIKDEDRTSLATFHEGKHNISHMSMDIARGLMVTCGTDRIVKIWDMTPVVGCSLATGFSPH Predict reactive species Full Name: WD repeat and FYVE domain containing 1 Calculated Molecular Weight: 410 aa, 46 kDa Observed Molecular Weight: 43-46 kDa GenBank Accession Number: BC040525 Gene Symbol: WDFY1 Gene ID (NCBI): 57590 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IWB7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.