Proteintech, 13961-1-AP, PPFIBP1 Polyclonal antibody

Size: 20ul / 150ul The PPFIBP1 (13961-1-AP) by Proteintech is a Polyclonal antibody targeting PPFIBP1 in WB, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human samples 13961-1-AP targets PPFIBP1 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, DU 145 cells Positive IP detected in: HepG2 cells Positive IF/ICC detected in: U2OS cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4884 Product name: Recombinant human PPFIBP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 662-1011 aa of BC050281 Sequence: GSYLNSGKHWIASGQTLLQASQQDLEKELGIKHSLHRKKLQLALQALGSEEETNHGKLDFNWVTRWLDDIGLPQYKTQFDEGRVDGRMLHYMTVDDLLSLKVVSVLHHLSIKRAIQVLRINNFEPNCLRRRPSDENTIAPSEVQKWTNHRVMEWLRSVDLAEYAPNLRGSGVHGGLMVLEPRFNVETMAQLLNIPPNKTLLRRHLATHFNLLIGAEAQHQKRDAMELPDYVLLTATAKVKPKKLAFSNFGNLRKKKQEDGEEYVCPMELGQASGSASKKGFKPGLDMRLYEEDDLDRLEQMEDSEGTVRQIGAFSEGINNLTHMLKEDDMFKDFAARSPSASITDEDSNV Predict reactive species Full Name: PTPRF interacting protein, binding protein 1 (liprin beta 1) Calculated Molecular Weight: 1011 aa, 114 kDa Observed Molecular Weight: 114 kDa GenBank Accession Number: BC050281 Gene Symbol: PPFIBP1 Gene ID (NCBI): 8496 RRID: AB_2171631 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86W92 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.