Proteintech, 14010-1-AP, SOX6 Polyclonal antibody

Size: 20ul / 150ul The SOX6 (14010-1-AP) by Proteintech is a Polyclonal antibody targeting SOX6 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 14010-1-AP targets SOX6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: U-251 cells, Jurkat cells, C6 cells Positive IHC detected in: mouse testis tissue, mouse skin tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information The Sex-determining Region Y (SRY)-related high-mobility group box 6 (SOX6) is a transcription factor that was discovered in 1990s while searching for Sry-related genes. SOX6 belongs to the subfamily D of SOX protein and can recognize the conserved sequence of "(A/T)(A/T)CAA(A/ T)G" within the transcriptional regulation region of the target gene with its HMG domain. Sox6 contains DNA binding domain and binds to the minor groove of DNA. The ability of SOX6 to interact with different proteins at different places and times, makes it an intricate protein with roles in multiple processes. SOX6 has been implicated in diabetic nephropathy, adipogenesis, cardiomyopathy, inflammatory bowel disease, and cancer. (PMID: 33230925) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5111 Product name: Recombinant human SOX6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 272-828 aa of BC047064 Sequence: FPHDQRTLAAAAAAQQGFLFPPGITYKPGDNYPVQFIPSTMAAAAASGLSPLQLQKGHVSHPQINQRLKGLSDRFGRNLDTFEHGGGHSYNHKQIEQLYAAQLASMQVSPGAKMPSTPQPPNTAGTVSPTGIKNEKRGTSPVTQVKDEAAAQPLNLSSRPKTAEPVKSPTSPTQNLFPASKTSPVNLPNKSSIPSPIGGSLGRGSSLDILSSLNSPALFGDQDTVMKAIQEARKMREQIQREQQQQQPHGVDGKLSSINNMGLNSCRNEKERTRFENLGPQLTGKSNEDGKLGPGVIDLTRPEDAEGSKAMNGSAAKLQQYYCWPTGGATVAEARVYRDARGRASSEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKSMSNQEKQPYYEEQARLSKIHLEKYPNYKYKPRPKRTCIVDGKKLRIGEYKQLMRSRRQEMRQFFTVGQQPQIPITTGTGVVYPGAITMATTTPSPQMTSDCSSTSASPEPSLPVIQSTYGMKTDGGSLAGNEMINGEDEMEMYDDYEDDPKSDYSSENEAPEAVSAN Predict reactive species Full Name: SRY (sex determining region Y)-box 6 Calculated Molecular Weight: 92 kDa Observed Molecular Weight: 90 kDa GenBank Accession Number: BC047064 Gene Symbol: SOX6 Gene ID (NCBI): 55553 RRID: AB_2196095 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P35712 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.