Proteintech, 14022-1-AP, PSMA8 Polyclonal antibody

Size: 20ul / 150ul The PSMA8 (14022-1-AP) by Proteintech is a Polyclonal antibody targeting PSMA8 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 14022-1-AP targets PSMA8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, K-562 cells, mouse thymus tissue, HeLa cells, rat thymus tissue Positive IHC detected in: human testis tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:200-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5138 Product name: Recombinant human PSMA8 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-250 aa of BC042820 Sequence: MASRYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGIRGTNIVVLGVEKKSVAKLQDERTVRKICALDDHVCMAFAGLTADARVVINRARVECQSHKLTVEDPVTVEYITRFIATLKQKYTQSNGRRPFGISALIVGFDDDGISRLYQTDPSGTYHAWKANAIGRSAKTVREFLEKNYTEDAIASDSEAIKLAIKALLEVVQSGGKNIELAIIRRNQPLKMFSAKEVELYVTEIEKEKEEAEKKKSKKSV Predict reactive species Full Name: proteasome (prosome, macropain) subunit, alpha type, 8 Calculated Molecular Weight: 29 kDa Observed Molecular Weight: 28 kDa GenBank Accession Number: BC042820 Gene Symbol: PSMA8 Gene ID (NCBI): 143471 RRID: AB_10896314 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8TAA3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.