Proteintech, 14054-1-AP, DLK/MAP3K12 Polyclonal antibody

Size: 20ul / 150ul The DLK/MAP3K12 (14054-1-AP) by Proteintech is a Polyclonal antibody targeting DLK/MAP3K12 in WB, ELISA applications with reactivity to human samples 14054-1-AP targets DLK/MAP3K12 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, Calu-1 cells, HL-60 cells, MCF-7 cells, MDA-MB-231 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Background Information MAP3K12, also named as DLK ((dual leucine zipper kinase), is a part of a non-canonical MAPK signaling pathway. It has been reported that MAP3K12 can be activated by APOE through a receptor-mediated mechanism. MAP3K12 enhances the AP-1-mediated transcription of APP via a MAP kinase signal transduction pathway composed of MAP2K7, MAPK1/ERK2 and MAPK3/ERK1. MAP3K12 is required for pancreatic beta-cell proliferation during postnatal development and stress-induced JNK signaling in neurons.(PMID: 28111074, 25341110, 32189007) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4780 Product name: Recombinant human MAP3K12 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 220-567 aa of BC050050 Sequence: CILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWREEVKLHFEKIKSEGTCLHRLEEELVMRRREELRHALDIREHYERKLERANNLYMELNALMLQLELKERELLRREQALERRCPGLLKPHPSRGPQLPSLHESHSYSLYIEKYLYKLYIYTYIYIYAPHNQQKDGAVPAVSQARGRLIP Predict reactive species Full Name: mitogen-activated protein kinase kinase kinase 12 Calculated Molecular Weight: 567 aa, 64 kDa Observed Molecular Weight: 100-110 kDa GenBank Accession Number: BC050050 Gene Symbol: MAP3K12 Gene ID (NCBI): 7786 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q12852 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.