Product Description
Size: 20ul / 150ul
The DOK2 (14087-1-AP) by Proteintech is a Polyclonal antibody targeting DOK2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples
14087-1-AP targets DOK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: Jurkat cells
Positive IHC detected in: human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:500-1:2400
Immunohistochemistry (IHC): IHC : 1:20-1:200
Background Information
Docking protein 2 (Dok2) is an adapter protein which is involved in hematopoiesis. Dok2 is able to control Klf1 expression by transcriptional regulation through directly binding to its promoter region (PMID: 25075100).
Specification
Tested Reactivity: human, mouse, rat
Cited Reactivity: human
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Immunogen: CatNo: Ag5218 Product name: Recombinant human DOK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-412 aa of BC032623 Sequence: MGDGAVKQGFLYLQQQQTFGKKWRRFGASLYGGSDCALARLELQEGPEKPRRCEAARKVIRLSDCLRVAEAGGEASSPRDTSAFFLETKERLYLLAAPAAERGDWVQAICLLAFPGQRKELSGPEGKQSRPCMEENELYSSAVTVGPHKEFAVTMRPTEASERCHLRGSYTLRAGESALELWGGPEPGTQLYDWPYRFLRRFGRDKVTFSFEAGRRCVSGEGNFEFETRQGNEIFLALEEAISAQKNAAPATPQPQPATIPASLPRPDSPYSRPHDSLPPPSPTTPVPAPRPRGQEGEYAVPFDAVARSLGKNFRGILAVPPQLLADPLYDSIEETLPPRPDHIYDEPEGVAALSLYDSPQEPRGEAWRRQATADRDPAGLQHVQPAGQDFSASGWQPGTEYDNVVLKKGPK Predict reactive species
Full Name: docking protein 2, 56kDa
Calculated Molecular Weight: 412 aa, 45 kDa
Observed Molecular Weight: 56 kDa
GenBank Accession Number: BC032623
Gene Symbol: DOK2
Gene ID (NCBI): 9046
RRID: AB_2230792
Conjugate: Unconjugated
Form: Liquid
Purification Method: Antigen affinity purification
UNIPROT ID: O60496
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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