Proteintech, 14104-1-AP, GABRG2 Polyclonal antibody

Size: 20ul / 150ul The GABRG2 (14104-1-AP) by Proteintech is a Polyclonal antibody targeting GABRG2 in WB, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples 14104-1-AP targets GABRG2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Neuro-2a cells, mouse brain tissue Positive IP detected in: mouse brain tissue Positive IF-P detected in: mouse cerebellum tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information GABRG2 (gamma-aminobutyric acid type A receptor subunit gamma2), which is expected to be located in cell membrane and expressed in brain and retina. The calculated molecular weight of the protein is 54 kDa. This antibody can recognize the 43 kDa band of target (PMID:12091434). And it also has glycosylation modification. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5237 Product name: Recombinant human GABRG2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 42-274 aa of BC059389 Sequence: SDDDYEDYASNKTWVLTPKVPEGDVTVILNNLLEGYDNKLRPDIGVKPTLIHTDMYVNSIGPVNAINMEYTIDIFFAQTWYDRRLKFNSTIKVLRLNSNMVGKIWIPDTFFRNSKKADAHWITTPNRMLRIWNDGRVLYTLRLTIDAECQLQLHNFPMDEHSCPLEFSSYGYPREEIVYQWKRSSVEVGDTRSWRLYQFSFVGLRNTTEVVKTTSGDYVVMSVYFDLSRRMGY Predict reactive species Full Name: gamma-aminobutyric acid (GABA) A receptor, gamma 2 Calculated Molecular Weight: 467 aa, 54 kDa Observed Molecular Weight: 54-60 kDa GenBank Accession Number: BC059389 Gene Symbol: GABRG2 Gene ID (NCBI): 2566 RRID: AB_10693527 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P18507 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.