Proteintech, 14114-1-AP, ATP5J Polyclonal antibody

Size: 20ul / 150ul The ATP5J (14114-1-AP) by Proteintech is a Polyclonal antibody targeting ATP5J in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 14114-1-AP targets ATP5J in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HUVEC cells, mouse liver tissue, human heart tissue, SKOV-3 cells, mouse heart tissues, rat heart tissues Positive IP detected in: HEK-293 cells Positive IHC detected in: human osteosarcoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, U-251 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information ATP5J, also known as coupling factor 6 (CF6), is a soluble integral component of mitochondrial ATP synthase. Mitochondrial ATP synthase is a multi-subunit membrane-bound enzyme that catalyzes the synthesis of ATP by utilizing a proton electrochemical gradient. It consists of three domains, namely the extrinsic and intrinsic membrane domains (F1 and F0, respectively) joined by a stalk. CF6 is one of the subunits in the stalk and an essential component for energy transduction. Recently CF6 has also been reported to play a crucial role in the development of INS resistance and hypertension. CF6 is first synthesized as an immature form in the cytosol, then transported to the mitochondria by an import signal peptide and becomes an active form with the signal peptide cleaved. Western blot analysis of CF6 demonstrates a single band around 9 kDa to 12 kDa in various tissues including heart, liver, brain and HUVEC (human umbilical vein endothelial cells). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, rabbit Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5263 Product name: Recombinant human ATP5J protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-108 aa of BC066310 Sequence: MILQRLFRFSSVIRSAVSVHLRRNIGVTAVAFNKELDPIQKLFVDKIREYKSKRQTSGGPVDASSEYQQELERELFKLKQMFGNADMNTFHTFKFEDPKFEVIEKPQA Predict reactive species Full Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 Calculated Molecular Weight: 13 kDa Observed Molecular Weight: 9 kDa GenBank Accession Number: BC066310 Gene Symbol: ATP5J Gene ID (NCBI): 522 RRID: AB_2062056 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P18859 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.