Proteintech, 14132-1-AP, GFPT1 Polyclonal antibody

Size: 20ul / 150ul The GFPT1 (14132-1-AP) by Proteintech is a Polyclonal antibody targeting GFPT1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 14132-1-AP targets GFPT1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human skeletal muscle tissue, HepG2 cells Positive IHC detected in: human gliomas tissue, human hepatocirrhosis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information GFPT1 (glutamine-fructose-6-phosphate transaminase 1) catalyzes the transfer of an amino group from glutamine onto fructose-6-phosphate, yielding glucosamine-6-phosphate (GlcN-6-P) and glutamate. GFPT1 is the key enzyme of the hexosamine pathway and is required for critical events in neuromuscular transmission. Mutations of GFPT1 cause congenital myasthenic syndromes (CMS). (21310273, 25765662) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5312 Product name: Recombinant human GFPT1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC045641 Sequence: MCGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDGGNDKDWEANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHGEPSPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTETIAKLVKYMYDNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVGTRRGSPLLIGVRSEHKLSTDHIPILYRTGKDKKGSCNLSRVDSTTCLFPVEEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGDHPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYT Predict reactive species Full Name: glutamine-fructose-6-phosphate transaminase 1 Calculated Molecular Weight: 681 aa, 77 kDa Observed Molecular Weight: 77 kDa GenBank Accession Number: BC045641 Gene Symbol: GFPT1 Gene ID (NCBI): 2673 RRID: AB_2110155 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q06210 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.