Proteintech, 14142-1-AP, ISYNA1 Polyclonal antibody

Size: 20ul / 150ul The ISYNA1 (14142-1-AP) by Proteintech is a Polyclonal antibody targeting ISYNA1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 14142-1-AP targets ISYNA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, HepG2 cells, rat testis tissue Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information Myo-inositol 3-phosphate synthase (ISYNA1/IP synthase) is a rate-limiting enzyme that catalyzes the first step in the biosynthesis of all inositol containing compounds. It converts glucose 6-phosphate to Myo-inositol 3-phosphate.(PMID:21841945). The native enzyme is typically a homotrimermade up of 68-kDa subunits in mammalian cells and a homotetramer in yeast and plant cells. There are three new isoforms of 62, 43, and 16kDa detected suggesting ISYNA1 is a completely different holoenzyme. But only the brain and testis manifest the 68-kDa isoform, whereas the pancreas has a slightly smaller isoformat 67 kDa. The intestine has a completely unique isoformprofile comprising 62,43, 20kDa isoforms(PMID:19188364). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5272 Product name: Recombinant human ISYNA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 209-558 aa of BC066902 Sequence: RDIRDFRSSAGLDKVIVLWTANTERFCEVIPGLNDTAENLLRTIELGLEVSPSTLFAVASILEGCAFLNGSPQNTLVPGALELAWQHRVFVGGDDFKSGQTKVKSVLVDFLIGSGLKTMSIVSYNHLGNNDGENLSAPLQFRSKEVSKSNVVDDMVQSNQVLYTPGEEPDHCVVIKYVPYVGDGKRALDEYTSELMLGGTNTLVLHNTCEDSLLAAPIMLDLALLTELCQRVSFCTDMDPEPQTFHPVLSLLSFLFKAPLVPRGSPVVNALFRQRSCIENILRACVGLPPQNHMLLEHKMERPGPSLKRVGPVAATYPMLNKKGPVPAATNGCTGDANGHLQEEPPMPTT Predict reactive species Full Name: inositol-3-phosphate synthase 1 Calculated Molecular Weight: 61 kDa Observed Molecular Weight: 61 kDa GenBank Accession Number: BC066902 Gene Symbol: ISYNA1 Gene ID (NCBI): 51477 RRID: AB_2233801 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NPH2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.