Proteintech, 14242-1-AP, PIAS4 Polyclonal antibody

Size: 20ul / 150ul The PIAS4 (14242-1-AP) by Proteintech is a Polyclonal antibody targeting PIAS4 in WB, ELISA applications with reactivity to human, mouse samples 14242-1-AP targets PIAS4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A375 cells, HL-60 cells, K-562 cells, mouse ovary tissue, mouse pancreas tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members (PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5498 Product name: Recombinant human PIAS4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 210-510 aa of BC029874 Sequence: PNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIRAEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMENGKPGADVVDLTLDSSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVPAC Predict reactive species Full Name: protein inhibitor of activated STAT, 4 Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 57-70 kDa GenBank Accession Number: BC029874 Gene Symbol: PIAS4 Gene ID (NCBI): 51588 RRID: AB_2164365 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N2W9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.